Joseph Colarusso
Biomedical engineering
Hometown: Chula Vista, California, United States
Graduation date: Spring 2025
Additional details: Honors student
FURI | Spring 2024
Optimizing Cell Culture Methods for Acetylcholinesterase Production
The Clark Lab currently produces Acetylcholinesterase (AChE) for use in pH-responsive nanosensors in-house, by collecting waste media from transfected HEK293T cells in a medium with 10% Fetal Bovine Serum (FBS). Previous research has shown HEK293T cells to be less efficient in recombinant protein production than other lines such as CHO-K1 cells, and media containing FBS to diminish recombinant protein purity. This project investigates and compares the productivity of cultures following the labs current methods, using transfected CHO-K1 cells, and using HEK293T cells in a serum-free media to find the optimal cell culture methodology for AChE production.
Mentor: Heather Clark
It’s hip to be square.
Students presenting projects at the Fulton Forge Student Research Expo are encouraged to download this personal QR code and include it within your poster. This allows expo attendees to explore more about your project and about you in the future.
Right click the image to save it to your computer.