FURI | Spring 2019

Using 1H MRI Reporter S-Gal® to Study Senescent and Quiescent Cells

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Senescent and quiescent cells are cells that stop proliferating due to a variety of factors, including stress-induced senescence. Recognizing these cells through MR imaging can help detect dormant  cancer tumors. It has been shown that an enzyme called β-galactosidase (β-gal) is upregulated in senescent and quiescent cells.The combination of S-gal® and ferric ammonium citrate (FAC) in the presence of β-gal leads to the formation of a black, paramagnetic precipitate. This study uses NIH-3T3 cells (mouse fibroblast) and NCI-H1975 (human lung epithelial) cells cultured in vitro with the goal of detecting the black, paramagnetic precipitate through MR imaging and western blotting techniques.

Student researcher

Nyah Kshatriya

Nyah Kshatriya

Biomedical engineering

Hometown: Scottsdale, AZ, United States

Graduation date: Fall 2021