FURI | Fall 2022

Engineering Corynebacterium glutamicum with Malonate Pathway for Optimizing Flavonoid Production

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This project seeks to address the question: What is the effect of expressing a malonate pathway in C. glutamicum strains on naringenin output? Findings suggest the optimal MatC induction with MatB is between 100 µM to 1 mM, and ideal MatC induction with aTc is between 10–50 ng/mL. Furthermore, low product titer and yield suggested that the naringenin pathway expression may be unoptimized, so RT-PCRs were run to quantify RNA transcripts of the pathway genes. Higher MatB synthase and MatC protein transporter expression levels result in greater conversion of Malonyl-coA to Naringenin. Naringenin can be used as a supplement for farm animals, enabling them to produce healthier offspring and more nutritional products for human consumption at an accelerated rate to match the growing global demand for food.

Student researcher

Juhi Khandelwal

Chemical engineering

Hometown: Chandler, Arizona, United States

Graduation date: Spring 2025