MORE | Fall 2022

Transcriptomic Assays of Immunometabolic Enhancers

Aging-related diseases are primarily metabolic, leading to long-term inflammation, but in drug discovery, immunometabolism remains difficult to assess and often relies on expensive and low throughput methods like mass spectroscopy or transcriptomics. This project will establish real-time fluorescence assays that model and measure neuroinflammatory response to stimuli in human microglial cells. Fluorescence will be induced using transcription factors known to play a role in neuroinflammation: nuclear factor kappa B (NF-kB) and/or peroxisome proliferator activated receptor (PPAR). Fluorescence will be analyzed and used to quantify cell inflammatory response to stimuli.