MORE | Spring 2025

Automating Reagent Production Process for Use in Microfluidic Chips

Point-of-care diagnostic devices enable rapid disease detection but can require labor-intensive fabrication. Specifically, the lyophilization (freeze-drying) of reagents is a complex but necessary process that preserves the shape and enzymatic activity of reagents for use in the reaction chambers of microfluidic chips. This study aims to automate the lyophilization process by designing a 3D-printed rig to eject and transfer frozen reagent shapes. The device was evaluated for efficiency and reagent integrity, with a goal of reducing handling time, improving reproducibility, and minimizing reagent damage. These advancements enhance diagnostic device fabrication and improve accessibility to rapid and cost-effective disease testing.