FURI | Fall 2020

Assessment of Injury Induced Blood Brain Barrier Disruption and Necroptosis via Development of Rapid Data Analysis Pipeline

Previous modes of image analysis were determined to be unfit for efficient usage in laboratory analysis tasks. Improvements were made upon the Java application created during Summer 2020 FURI, including heightened automation ability, cell counting, and overlaid signal characterization. The precision tools included in the customizable program allow for time efficient and accurate analysis of colorimetric (i.e. horseradish peroxidase) and fluorescence (i.e. immunofluorescence) staining in tissue samples. The analysis completed using this program allowed for the characterization of neurodegeneration in post TBI mice brain tissue.